PPP1R3B antibody (AA 1-285)

Catalog No. ABIN7599215

The Rabbit Polyclonal anti-PPP1R3B antibody has been validated for WB and ELISA. It is suitable to detect PPP1R3B in samples from Human.

Quick Overview for PPP1R3B antibody (AA 1-285) (ABIN7599215)

Target: PPP1R3B (Protein Phosphatase 1, Regulatory Subunit 3B (PPP1R3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PPP1R3B Antibody

Binding Specificity: AA 1-285

Purpose: Anti-PPP1R3B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PPP1R3B Antibody Picoband® (ABIN7599215). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPP1R3B recombinant protein (Position: M1-Y285).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Doherty, M. J., Moorhead, G., Morrice, N., Cohen, P., Cohen, P. T. W. Amino acid sequence and expression of hepatic glycogen-binding (G-L)-subunit of protein phosphatase-1. FEBS Lett. 375: 294-298, 1995. 2. Dunn, J. S., Mlynarski, W. M., Pezzoles, M. G., Borowiec, M., Powers, C., Krolewski, A. S., Doria, A. Examination of PPP1R3B as a candidate gene for the type 2 diabetes and MODY loci on chromosome 8p23. Ann. Hum. Genet. 70: 587-593, 2006. 3. Munro, S., Cuthbertson, D. J. R., Cunningham, J., Sales, M., Cohen, P. T. W. Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G-L of liver. Diabetes 51: 591-598, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPP1R3B

Target: PPP1R3B (Protein Phosphatase 1, Regulatory Subunit 3B (PPP1R3B))

Alternative Name: PPP1R3B

Background:

Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: This gene encodes the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The encoded protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein.

Molecular Weight: 33 kDa

Gene ID: 79660

Pathways: Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process