CrkL antibody (AA 1-290)

Catalog No. ABIN7599222

The Rabbit Polyclonal anti-CrkL antibody (ABIN7599222) specifically detects CrkL in WB, ELISA, IF, ICC, IP and FACS. The antibody is reactive with Human samples.

Quick Overview for CrkL antibody (AA 1-290) (ABIN7599222)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-CrkL Antibody

Binding Specificity: AA 1-290

Purpose: Anti-CRKL Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CRKL Antibody (ABIN7599222). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CRKL recombinant protein (Position: M1-H290).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL

Background:

Synonyms: Periplakin,190 kDa paraneoplastic pemphigus antigen,195 kDa cornified envelope precursor protein,PPL,KIAA0568,

Tissue Specificity: Expressed in stratified squamous epithelia and in some other epithelia. .

Background: Crk-like protein is a protein that in humans is encoded by the CRKL gene. This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Molecular Weight: 34 kDa

Gene ID: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor