POLR1E antibody (AA 1-291)

Catalog No. ABIN7599225

This anti-POLR1E antibody is a Rabbit Polyclonal antibody detecting POLR1E in WB, ELISA, IP, IF and ICC. Suitable for Human.

Quick Overview for POLR1E antibody (AA 1-291) (ABIN7599225)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR1E Antibody

Binding Specificity: AA 1-291

Purpose: Anti-POLR1E Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-POLR1E Antibody Picoband® (ABIN7599225). Tested in WB, ICC/IF, IP, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLR1E recombinant protein (Position: M1-Q291). Human POLR1E shares 78.9% amino acid (aa) sequence identity with mouse POLR1E.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
ELISA, 0.1-0.5 μg/mL
1. Hanada, K., Song, C. Z., Yamamoto, K., Yano, K., Maeda, Y., Yamaguchi, K., Muramatsu, M. RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription. EMBO J. 15: 2217-2226, 1996. 2. Kong, R., Zhang, L., Hu, L., Peng, Q., Han, W., Du, X., Ke, Y. hALP, a novel transcriptional U three protein (t-UTP), activates RNA polymerase I transcription by binding and acetylating the upstream binding factor (UBF). J. Biol. Chem. 286: 7139-7148, 2011. 3. McNamar, R., Abu-Adas, Z., Rothblum, K., Knutson, B. A., Rothblum, L. I. Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains. J. Biol. Chem. 294: 19907-19922, 2019.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E

Background:

Synonyms: POLR1E, PAF53, PRAF1, DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53

Background: Predicted to enable DNA binding activity and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I and transcription initiation at RNA polymerase I promoter. Located in fibrillar center and nucleoplasm. Part of RNA polymerase I complex.

Molecular Weight: 47 kDa

Gene ID: 64425