POLR1E antibody (N-Term)

Catalog No. ABIN2787316

The Rabbit Polyclonal anti-POLR1E antibody is suitable to detect POLR1E in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for POLR1E antibody (N-Term) (ABIN2787316)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR1E Antibody

Binding Specificity: N-Term

Purpose: POLR1E Antibody - N-terminal region

Sequence: SPGNMRFTLY ENKDSTNPRK RNQRILAAET DRLSYVGNNF GTGALKCNTL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against POLR1E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR1E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP57639-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E

Background:

Targeted Subunit: RPA49

Background Information: POLR1E is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.POLR1E is the component of RNA polymerase I which synthesizes ribosomal RNA precursors.POLR1E appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.

Gene Name: Polymerase (RNA) I polypeptide E, 53 kDa

Alternative Symbols: A49, PAF53, PRAF1, RPA49

Protein Name: DNA-directed RNA polymerase I subunit RPA49

Molecular Weight: 47kDa

Gene ID: 64425

NCBI Accession: NP_071935

UniProt: Q9GZS1