PTF1A antibody (AA 1-292)

Catalog No. ABIN7599226

This Rabbit Polyclonal antibody specifically detects PTF1A in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PTF1A antibody (AA 1-292) (ABIN7599226)

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PTF1A Antibody

Binding Specificity: AA 1-292

Purpose: Anti-PTF1A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PTF1A Antibody Picoband® (ABIN7599226). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PTF1A recombinant protein (Position: M1-Q292).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Al-Shammari, M., Al-Husain, M., Al-Kharfy, T., Alkuraya, F. S. A novel PTF1A mutation in a patient with severe pancreatic and cerebellar involvement. (Letter) Clin. Genet. 80: 196-198, 2011. 2. Sellick, G. S., Barker, K. T., Stolte-Dijkstra, I., Fleischmann, C., Coleman, R. J., Garrett, C., Gloyn, A. L., Edghill, E. L., Hattersley, A. T., Wellauer, P. K., Goodwin, G., Houlston, R. S. Mutations in PTF1A cause pancreatic and cerebellar agenesis. Nature Genet. 36: 1301-1305, 2004. 3. Weedon, M. N., Cebola, I., Patch, A.-M., Flanagan, S. E., De Franco, E., Caswell, R., Rodriguez-Segui, S. A., Shaw-Smith, C., Cho, C. H.-H., Allen, H. L., Houghton, J. A. L., Roth, C. L., and 9 others. Recessive mutations in a distal PTF1A enhancer cause isolated pancreatic agenesis. Nature Genet. 46: 61-64, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PTF1A

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Alternative Name: PTF1A

Background:

Synonyms: Pancreas transcription factor 1 subunit alpha, Class A basic helix-loop-helix protein 29, bHLHa29, Pancreas-specific transcription factor 1a, bHLH transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1, PTF1-p48, PTF1A, BHLHA29, PTF1P48

Tissue Specificity: Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.

Background: Pancreas transcription factor 1 subunit alpha is a protein that in humans is encoded by the PTF1A gene. It is mapped to 10p12.2. This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.

Molecular Weight: 48 kDa

Gene ID: 256297

Pathways: Retinoic Acid Receptor Signaling Pathway