CD20 antibody (AA 1-297)

Catalog No. ABIN7599234

This anti-CD20 antibody is a Mouse Monoclonal antibody detecting CD20 in WB and IF. Suitable for Human.

Quick Overview for CD20 antibody (AA 1-297) (ABIN7599234)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 4I11

Product Details anti-CD20 Antibody

Binding Specificity: AA 1-297

Purpose: Anti-CD20/MS4A1 Antibody Picoband® (monoclonal, 4I11)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD20/MS4A1 Antibody Picoband® (monoclonal, 4I11) (ABIN7599234). Tested in IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD20/MS4A1 recombinant protein (Position: M1-P297).

Isotype: IgG2a

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
1. Cragg MS, Walshe CA, Ivanov AO, Glennie MJ (2005). "The biology of CD20 and its potential as a target for mAb therapy". Curr. Dir. Autoimmun. Current ions in Autoimmunity 8: 140-74. 2. Hardy, Richard (2008). "Chapter 7: B Lymphocyte Development and Biology". In Paul, William.Fundamental Immunology (Book) (6th ed.). Philadelphia: Lippincott Williams & Wilkins. pp. 237-269. 3. Kuijpers TW, Bende RJ, Baars PA, Grummels A, Derks IA, Dolman KM, Beaumont T, Tedder TF, van Noesel CJ, Eldering E, van Lier RA (January 2010). "CD20 deficiency in humans results in impaired T cell-independent antibody responses". J. Clin. Invest. 120 (1): 214-22.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: MS4A1

Background:

Synonyms: Eukaryotic translation initiation factor 6, eIF-6, B (2)GCN homolog, B4 integrin interactor, CAB, p27 (BBP), EIF6, EIF3A, ITGB4BP, OK/SW-cl.27

Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle.

Background: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

Molecular Weight: 37 kDa

Gene ID: 931

UniProt: P11836