TOMM34 antibody (AA 1-309)

Catalog No. ABIN7599253

This Rabbit Polyclonal antibody specifically detects TOMM34 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TOMM34 antibody (AA 1-309) (ABIN7599253)

Target: TOMM34 (Translocase of Outer Mitochondrial Membrane 34 (TOMM34))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOMM34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-TOMM34 Antibody

Binding Specificity: AA 1-309

Purpose: Anti-TOMM34 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TOMM34 Antibody Picoband® (ABIN7599253). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TOMM34 recombinant protein (Position: M1-H309).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Faou, P., Hoogenraad, N. J. Tom34: a cytosolic cochaperone of the Hsp90/Hsp70 protein complex involved in mitochondrial protein import. Biochim. Biophys. Acta 1823: 348-357, 2012. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 10/8/2014. 3. Mukhopadhyay, A., Avramova, L. V., Weiner, H. Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins. Arch. Biochem. Biophys. 400: 97-104, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TOMM34

Target: TOMM34 (Translocase of Outer Mitochondrial Membrane 34 (TOMM34))

Alternative Name: TOMM34

Background:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: Mitochondrial import receptor subunit TOM34 is a protein that in humans is encoded by the TOMM34 gene. The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats.

Molecular Weight: 35 kDa

Gene ID: 10953

UniProt: Q15785