NUBP1 antibody (AA 1-311)

Catalog No. ABIN7599255

This anti-NUBP1 antibody is a Rabbit Polyclonal antibody detecting NUBP1 in WB, ELISA, IF, FACS and ICC. Suitable for Human.

Quick Overview for NUBP1 antibody (AA 1-311) (ABIN7599255)

Target: NUBP1 (Nucleotide Binding Protein 1 (NUBP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NUBP1 Antibody

Binding Specificity: AA 1-311

Purpose: Anti-NUBP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUBP1 Antibody Picoband® (ABIN7599255). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUBP1 recombinant protein (Position: M1-Q311).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/11/2012. 2. Nakashima, H., Grahovac, M. J., Mazzarella, R., Fujiwara, H., Kitchen, J. R., Threat, T. A., Ko, M. S. H. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to the chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. Genomics 60: 152-160, 1999. 3. Shahrestanifar, M., Saha, D. P., Scala, L. A., Basu, A., Howells, R. D. Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD. Gene 147: 281-285, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NUBP1

Target: NUBP1 (Nucleotide Binding Protein 1 (NUBP1))

Alternative Name: NUBP1

Background:

Synonyms: PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, PHF21A, BHC80, KIAA1696, BM-006

Tissue Specificity: Highly expressed in brain. Expressed at much lower level in other tissues.

Background: NUBP1 also known as NBP as is expressed in several tissues with the highest being in the lung and testis. NUBP1 appears to interact with actin and the cytoskeleton and is required for centrosome duplication but it is also need for proper synthesis of iron sulfur proteins and the protein also binds ATP. NUBP1 has roles in cell growth, cellular iron homeostasis, centrosome localization, iron-sulfur cluster assembly, and apolipoprotein modification. NUBP1 is localized to the cytoplasm. Interestingly too, recent research indicates that mutations in NUBP1 in mice lead defects in lung development and eye cataracts, many of which are traced back to NUBP1 role in regulating centrosome dynamics and microtubule organization.

Molecular Weight: 35 kDa

Gene ID: 4682

UniProt: P53384

Pathways: Transition Metal Ion Homeostasis