LHX1 antibody (AA 1-317)

Catalog No. ABIN7599263

The Rabbit Polyclonal anti-LHX1 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect LHX1 in samples from Human and Mouse.

Quick Overview for LHX1 antibody (AA 1-317) (ABIN7599263)

Target: LHX1 (LIM Homeobox 1 (LHX1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LHX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-LHX1 Antibody

Binding Specificity: AA 1-317

Purpose: Anti-LIM1/LHX1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LIM1/LHX1 Antibody Picoband® (ABIN7599263). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LIM1/LHX1 recombinant protein (Position: M1-S317).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bozzi, F., Bertuzzi, S., Strina, D., Giannetto, C., Vezzoni, P., Villa, A. The exon-intron structure of human LHX1 gene. Biochem. Biophys. Res. Commun. 229: 494-497, 1996. 2. Dong, W. F., Heng, H. H., Lowsky, R., Xu, Y., DeCoteau, J. F., Shi, X. M., Tsui, L. C., Minden, M. D. Cloning, expression, and chromosomal localization to 11p12-13 of a human LIM/HOMEOBOX gene, hLim-1. DNA Cell Biol. 16: 671-678, 1997. 3. Dorus, S., Vallender, E. J., Evans, P. D., Anderson, J. R., Gilbert, S. L., Mahowald, M., Wyckoff, G. J., Malcom, C. M., Lahn, B. T. Accelerated evolution of nervous system genes in the origin of Homo sapiens. Cell 119: 1027-1040, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LHX1

Target: LHX1 (LIM Homeobox 1 (LHX1))

Alternative Name: LHX1

Background:

Synonyms: LIM/homeobox protein Lhx1, LIM homeobox protein 1, Homeobox protein Lim-1, hLim-1, LHX1, LIM-1, LIM1

Tissue Specificity: Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58 % of acute myeloid leukemia (AML) cell lines.

Background: LIM homeobox 1 is a protein that in humans is encoded by the LHX1 gene. It is mapped to 17q12. This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract.

Molecular Weight: 43 kDa

Gene ID: 3975

UniProt: P48742