Septin 2 antibody (AA 1-326)

Catalog No. ABIN7599275

The Rabbit Polyclonal anti-Septin 2 antibody has been validated for WB, ELISA, IHC, IF, FACS and ICC. It is suitable to detect Septin 2 in samples from Human, Mouse, Rat and Monkey.

Quick Overview for Septin 2 antibody (AA 1-326) (ABIN7599275)

Target: Septin 2 (SEPT2)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Septin 2 Antibody

Binding Specificity: AA 1-326

Purpose: Anti-Septin 2/SEPTIN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Septin 2/SEPTIN2 Antibody Picoband® (ABIN7599275). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Septin 2/SEPTIN2 recombinant protein (Position: M1-E326).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Xu C et al. Coupling function of cyclin-dependent kinase 2 and Septin2 in the promotion of hepatocellular carcinoma. Cancer Sci 110:540-549 (2019). 2. Omrane M et al. Septin 9 has Two Polybasic Domains Critical to Septin Filament Assembly and Golgi Integrity. iScience 13:138-153 (2019). 3. Akil A et al. Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV. Nat Commun 7:12203 (2016).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Septin 2

Target: Septin 2 (SEPT2)

Alternative Name: SEPTIN2

Background:

Synonyms: NADH-ubiquinone oxidoreductase chain 2, NADH dehydrogenase subunit 2, Mtnd2, mt-Nd2, Nd2

Tissue Specificity: Ubiquitously expressed.

Background: Septin 2, also known as SEPT2, is a protein which in humans is encoded by the SEPT2 gene. Enables identical protein binding activity. Predicted to be involved in several processes, including cilium assembly, regulation of exocytosis, and smoothened signaling pathway. Predicted to act upstream of or within regulation of L-glutamate import across plasma membrane and regulation of protein localization. Located in several cellular components, including cytoskeleton, photoreceptor connecting cilium, and sperm annulus. Part of septin complex.

Molecular Weight: 41 kDa

Gene ID: 4735

UniProt: Q15019