RNF2 antibody (AA 1-336)

Catalog No. ABIN7599286

This Rabbit Polyclonal antibody specifically detects RNF2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for RNF2 antibody (AA 1-336) (ABIN7599286)

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RNF2 Antibody

Binding Specificity: AA 1-336

Purpose: Anti-RING2/RING1B/RNF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RING2/RING1B/RNF2 Antibody Picoband® (ABIN7599286). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RING2/RING1B/RNF2 recombinant protein (Position: M1-K336).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: RNF2 ring finger protein 2". 2. Lee SJ, Choi JY, Sung YM, Park H, Rhim H, Kang S (August 2001). "E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme". FEBS Lett 503 (1): 61-4. 3. Tuckfield, Annabel, Clouston David R, Wilanowski Tomasz M, Zhao Lin-Lin, Cunningham John M, Jane Stephen M (March 2002). "Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors". Mol. Cell. Biol. (United States) 22 (6): 1936-46.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RNF2

Target: RNF2 (Ring Finger Protein 2 (RNF2))

Alternative Name: RNF2

Background:

Synonyms: Vinculin, Metavinculin, MV, VCL

Tissue Specificity: Metavinculin is muscle-specific.

Background: E3 ubiquitin-protein ligase RING2 is an enzyme that in humans is encoded by the RNF2 gene. Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Molecular Weight: 38 kDa

Gene ID: 6045

UniProt: Q99496