PAN2 antibody (AA 1-356)

Catalog No. ABIN7599306

This anti-PAN2 antibody is a Rabbit Polyclonal antibody detecting PAN2 in ELISA, WB and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for PAN2 antibody (AA 1-356) (ABIN7599306)

Target: PAN2 (PAN2 Poly(A) Specific Ribonuclease Subunit Homolog (PAN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAN2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PAN2 Antibody

Binding Specificity: AA 1-356

Purpose: Anti-PAN2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PAN2 Antibody Picoband® (ABIN7599306). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PAN2 recombinant protein (Position: M1-L356).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bett, J. S., Ibrahim, A. F. M., Garg, A. K., Kelly, V., Pedrioli, P., Rocha, S., Hay, R. T. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability. Biochem. J. 451: 185-194, 2013. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 04/21/2017. 3. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PAN2

Target: PAN2 (PAN2 Poly(A) Specific Ribonuclease Subunit Homolog (PAN2))

Alternative Name: PAN2

Background:

Synonyms: AF4/FMR2 family member 4,ALL1-fused gene from chromosome 5q31 protein,Protein AF-5q31,Major CDK9 elongation factor-associated protein,AFF4,AF5Q31, MCEF,HSPC092,

Tissue Specificity: Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. .

Background: PAB-dependent poly(A)-specific ribonuclease subunit 2 is an enzyme that in humans is encoded by the PAN2 gene. This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants.

Molecular Weight: 135 kDa

Gene ID: 9924

UniProt: Q504Q3