Phospholamban antibody (AA 1-36)

Catalog No. ABIN7599309

This anti-Phospholamban antibody is a Rabbit Polyclonal antibody detecting Phospholamban in ELISA, WB and IHC. Suitable for Human, Rat and Mouse.

Quick Overview for Phospholamban antibody (AA 1-36) (ABIN7599309)

Target: Phospholamban (PLN)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Phospholamban Antibody

Binding Specificity: AA 1-36

Purpose: Anti-Phospholamban/PLN Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Phospholamban/PLN Antibody Picoband® (ABIN7599309). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Phospholamban/PLN recombinant protein (Position: M1-C36).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Rodriguez P, Kranias EG (December 2005). "Phospholamban: a key determinant of cardiac function and dysfunction". Arch Mal Coeur Vaiss 98 (12): 1239-43. 2. McTiernan, C. F., Frye, C. S., Lemster, B. H., Kinder, E. A., Ogletree-Hughes, M. L., Moravec, C. S., Feldman, A. M. : The human phospholamban gene: structure and expression. J. Molec. Cell Cardiol. 31: 679-692, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLN

Background:

Synonyms: Allograft inflammatory factor 1,AIF-1,Ionized calcium-binding adapter molecule 1,Protein G1,AIF1,G1, IBA1,

Tissue Specificity: Detected in T-lymphocytes and peripheral blood mononuclear cells. .

Background: Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2+ pump in cardiac muscle and skeletal muscle cells. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban is also expressed in slow-twitch skeletal muscle and some smooth muscle cells. It is observed that human ventricle and quadriceps displayed high levels of phospholamban transcripts and proteins, with markedly lower expression observed in smooth muscles, while the right atrium also expressed low levels of phospholamban. The structure of the human phospholamban gene closely resembles that reported for chicken, rabbit, rat, and mouse. Comparison of the human to other mammalian phospholamban genes indicated a marked conservation of sequence for at least 217 bp upstream of the transcription start site.

Molecular Weight: 23 kDa

Gene ID: 5350

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity