NSUN4 antibody (AA 1-379)

Catalog No. ABIN7599331

Product Details anti-NSUN4 Antibody

Target: NSUN4 (NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4))

Binding Specificity: AA 1-379

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: Anti-NSUN4 Antibody Picoband®

Characteristics: Anti-NSUN4 Antibody Picoband® (ABIN7599331). Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSUN4 recombinant protein (Position: M1-K379). Human NSUN4 shares 86.3% amino acid (aa) sequence identity with mouse NSUN4.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Camara, Y., Asin-Cayuela, J., Park, C. B., Metodiev, M. D., Shi, Y., Ruzzenente, B., Kukat, C., Habermann, B., Wibom, R., Hultenby, K., Franz, T., Erdjument-Bromage, H., Tempst, P., Hallberg, B. M., Gustafsson, C. M., Larsson, N.-G. MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome. Cell Metab. 13: 527-539, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 8/30/2013. 3. Spahr, H., Habermann, B., Gustafsson, C. M., Larsson, N.-G., Hallberg, B. M. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proc. Nat. Acad. Sci. 109: 15253-15258, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSUN4

Target: NSUN4 (NOL1/NOP2/Sun Domain Family, Member 4 (NSUN4))

Alternative Name: NSUN4 (NSUN4 Products)

Background: NSUN4 belongsto a family of RNA 5-methylcytosine (m5C) methyltransferases(MTases).MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts withMTERF4 to facilitate mitoribosomal assembly. The calculatedMWof NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.

Molecular Weight: 40 kDa

Gene ID: 387338