CBX8 antibody (AA 1-389)

Catalog No. ABIN7599340

This Mouse Monoclonal antibody specifically detects CBX8 in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for CBX8 antibody (AA 1-389) (ABIN7599340)

Target: CBX8 (Chromobox Homolog 8 (CBX8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 8G7

Product Details anti-CBX8 Antibody

Binding Specificity: AA 1-389

Purpose: Anti-Cbx8 Antibody Picoband® (monoclonal, 8G7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Cbx8 Antibody Picoband® (monoclonal, 8G7) (ABIN7599340). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cbx8 recombinant protein (Position: M1-R389).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bardos, J. I., Saurin, A. J., Tissot, C., Duprez, E., Freemont, P. S. HPC3 is a new human Polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties. J. Biol. Chem. 275: 28785-28792, 2000. 2. Oza, J., Ganguly, B., Kulkarni, A., Ginjala, V., Yao, M., Ganesan, S. A novel role of chromodomain protein CBX8 in DNA damage response. J. Biol. Chem. 291: 22881-22893, 2016. Note: Erratum: J. Biol. Chem. 292: 761 only, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CBX8

Target: CBX8 (Chromobox Homolog 8 (CBX8))

Alternative Name: CBX8

Background:

Synonyms: 5'-AMP-activated protein kinase subunit beta-2, AMPK subunit beta-2, PRKAB2

Tissue Specificity: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Background: CBX8 functions as a transcriptional repressor and has a role in DNA damage response. This gene is mapped to chromosome 17q25.3 based on an alignment of the CBX8 sequence with the genomic sequence (GRCh38).

Molecular Weight: 45 kDa

Gene ID: 57332