RAD9A antibody (AA 1-389)

Catalog No. ABIN7599342

The Rabbit Polyclonal anti-RAD9A antibody (ABIN7599342) specifically detects RAD9A in WB, ELISA, IHC and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for RAD9A antibody (AA 1-389) (ABIN7599342)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-RAD9A Antibody

Binding Specificity: AA 1-389

Purpose: Anti-Rad9/Rad9a Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Rad9/Rad9a Antibody (ABIN7599342). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Rad9/Rad9a recombinant protein (Position: M1-G389).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: Rad9a

Background:

Synonyms: Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Erf1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 55-60 kDa

Gene ID: 19367

UniProt: Q9Z0F6

Pathways: Positive Regulation of Response to DNA Damage Stimulus