RAD9B antibody (AA 1-403)

Catalog No. ABIN7599360

Product Details anti-RAD9B Antibody

Target: RAD9B (RAD9 Homolog B (RAD9B))

Binding Specificity: AA 1-403

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9B antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Anti-Rad9b Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Rad9b Antibody Picoband® (ABIN7599360). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Rad9b recombinant protein (Position: M1-G403).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Dufault, V. M., Oestreich, A. J., Vroman, B. T., Karnitz, L. M. Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene. Genomics 82: 644-651, 2003. 2. Hopkins, K. M., Wang, X., Berlin, A., Hang, H., Thaker, H. M., Lieberman, H. B. Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue. Cancer Res. 63: 591-598, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RAD9B

Target: RAD9B (RAD9 Homolog B (RAD9B))

Alternative Name: Rad9b (RAD9B Products)

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: By coimmunoprecipitation of erythroleukemia cells transfected with RAD9B, it was found that RAD9B associated with 3 endogenous checkpoint proteins, HUS1, RAD1, and RAD17, possibly in a 9-1-1-like complex. RAD9B also interacted with transfected HUS1B. It was also found decreased abundance of RAD9B mRNA in testicular seminomas relative to normal tissue controls and other types of testicular tumors.

Molecular Weight: 60 kDa

Gene ID: 231724