ST6GAL1 antibody (AA 1-406)

Catalog No. ABIN7599361

This Rabbit Polyclonal antibody specifically detects ST6GAL1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ST6GAL1 antibody (AA 1-406) (ABIN7599361)

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ST6GAL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ST6GAL1 Antibody

Binding Specificity: AA 1-406

Purpose: Anti-CDw75/ST6GAL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CDw75/ST6GAL1 Antibody Picoband® (ABIN7599361). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CDw75/ST6GAL1 recombinant protein (Position: M1-C406).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Grundmann, U., Nerlich, C., Rein, T., Zettlmeissl, G. Complete cDNA sequence encoding human beta-galactoside alpha-2,6-sialyltransferase. Nucleic Acids Res. 18: 667 only, 1990. 2. Kitagawa, H., Paulson, J. C. Differential expression of five sialyltransferase genes in human tissues. J. Biol. Chem. 269: 17872-17878, 1994. 3. Nicholls, J. M., Chan, M. C. W., Chan, W. Y., Wong, H. K., Cheung, C. Y., Kwong, D. L. W., Wong, M. P., Chui, W. H., Poon, L. L. M., Tsao, S. W., Guan, Y., Peiris, J. S. M. Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract. Nature Med. 13: 147-149, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ST6GAL1

Target: ST6GAL1 (ST6 beta-Galactosamide alpha-2,6-Sialyltranferase 1 (ST6GAL1))

Alternative Name: ST6GAL1

Background:

Synonyms: Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3A

Tissue Specificity: Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Background: Beta-galactoside alpha-2,6-sialyltransferase 1 is an enzyme that in humans is encoded by the ST6GAL1 gene. This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants.

Molecular Weight: 47 kDa

Gene ID: 6480

UniProt: P15907