RAD18 antibody (AA 1-408)

Catalog No. ABIN7599364

This Rabbit Polyclonal antibody specifically detects RAD18 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RAD18 antibody (AA 1-408) (ABIN7599364)

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-RAD18 Antibody

Binding Specificity: AA 1-408

Purpose: Anti-RAD18 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RAD18 Antibody (ABIN7599364). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RAD18 recombinant protein (Position: M1-D408).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RAD18

Target: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Alternative Name: RAD18

Background:

Synonyms: Guanine deaminase, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, p51-nedasin, GDA, KIAA1258

Tissue Specificity: Expressed in lung carcinoma cell lines but not in normal lung tissues.

Background: E3 ubiquitin-protein ligase RAD18 is an enzyme that in humans is encoded by the RAD18 gene. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Molecular Weight: 75 kDa

Gene ID: 56852