ACTR3 antibody (AA 1-418)

Catalog No. ABIN7599368

The Rabbit Polyclonal anti-ACTR3 antibody has been validated for WB, ELISA and FACS. It is suitable to detect ACTR3 in samples from Human, Mouse and Rat.

Quick Overview for ACTR3 antibody (AA 1-418) (ABIN7599368)

Target: ACTR3 (ARP3 Actin-Related Protein 3 (ACTR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTR3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ACTR3 Antibody

Binding Specificity: AA 1-418

Purpose: Anti-Arp3/ACTR3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Arp3/ACTR3 Antibody Picoband® (ABIN7599368). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Arp3/ACTR3 recombinant protein (Position: M1-S418).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Kim, J., Lee, J. E., Heynen-Genel, S., Suyama, E., Ono, K., Lee, K., Ideker, T., Aza-Blanc, P., Gleeson, J. G. Functional genomic screen for modulators of ciliogenesis and cilium length. Nature 464: 1048-1051, 2010. 2. Machesky, L. M., Reeves, E., Wientjes, F., Mattheyse, F. J., Grogan, A., Totty, N. F., Burlingame, A. L., Hsuan, J. J., Segal, A. W. Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. Biochem. J. 328: 105-112, 1997. 3. Robinson, R. C., Turbedsky, K., Kaiser, D. A., Marchand, J.-B., Higgs, H. N., Choe, S., Pollard, T. D. Crystal structure of Arp2/3 complex. Science 294: 1679-1684, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ACTR3

Target: ACTR3 (ARP3 Actin-Related Protein 3 (ACTR3))

Alternative Name: ACTR3

Background:

Synonyms: Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K (+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42, MIG1

Tissue Specificity: Ubiquitously expressed.

Background: The specific function of this gene has not yet been determined, however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 47 kDa

Gene ID: 10096

UniProt: P61158

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization