Exopolyphosphatase PRUNE1 (PRUNE1) (AA 1-444) antibody

Catalog No. ABIN7599398

The Rabbit Polyclonal anti- antibody has been validated for ELISA, IF, WB, FACS and ICC. It is suitable to detect in samples from Human, Mouse and Rat.

Quick Overview for Exopolyphosphatase PRUNE1 (PRUNE1) (AA 1-444) antibody (ABIN7599398)

Target: Exopolyphosphatase PRUNE1 (PRUNE1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 1-444

Purpose: Anti-PRUNE1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRUNE1 Antibody Picoband® (ABIN7599398). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRUNE1 recombinant protein (Position: M1-Q444).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alhaddad, B., Schossig, A., Haack, T. B., Kovacs-Nagy, R., Braunisch, M. C., Makowski, C., Senderek, J., Vill, K., Muller-Felber, W., Strom, T. M., Krabichler, B., Freisinger, P., and 17 others. PRUNE1 deficiency: expanding the clinical and genetic spectrum. Neuropediatrics 49: 330-338, 2018. 2. Costain, G., Shugar, A., Krishnan, P., Mahmutoglu, S., Laughlin, S., Kannu, P. Homozygous mutation in PRUNE1 in an Oji-Cree male with a complex neurological phenotype. Am. J. Med. Genet. 173A: 740-743, 2017. 3. Diana, D., Smaldone, G., De Antonellis, P., Pirone, L., Carotenuto, M., Alonzi, A., Di Gaetano, S., Zollo, M., Pedone, E. M., Fattorusso, R. Mapping functional interaction sites of human prune C-terminal domain by NMR spectroscopy in human cell lysates. Chemistry 19: 12217-12220, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Exopolyphosphatase PRUNE1 (PRUNE1)

Alternative Name: PRUNE1

Background:

Synonyms: Protein Bop, BH3-only protein, Retrotransposon Gag-like protein 10, RTL10, BOP, C22orf29

Tissue Specificity: Ubiquitously expressed.

Background: This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants.

Molecular Weight: 60 kDa

Gene ID: 58497