CALCOCO2 antibody (AA 1-446)

Catalog No. ABIN7599400

This Mouse Monoclonal antibody specifically detects CALCOCO2 in WB, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CALCOCO2 antibody (AA 1-446) (ABIN7599400)

Target: CALCOCO2 (Calcium Binding and Coiled-Coil Domain 2 (CALCOCO2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CALCOCO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 9E2F2

Product Details anti-CALCOCO2 Antibody

Binding Specificity: AA 1-446

Purpose: Anti-NDP52/CALCOCO2 Antibody Picoband® (monoclonal, 9E2F2)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NDP52/CALCOCO2 Antibody Picoband® (monoclonal, 9E2F2) (ABIN7599400). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NDP52/CALCOCO2 recombinant protein (Position: M1-L446).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Korioth, F., Gieffers, C., Maul, G. G., Frey, J. Molecular characterization of NDP52, a novel protein of the nuclear domain 10, which is redistributed upon virus infection and interferon treatment. J. Cell Biol. 130: 1-13, 1995. 2. Lazarou, M., Sliter, D. A., Kane, L. A., Sarraf, S. A., Wang, C., Burman, J. L., Sideris, D. P., Fogel, A. I., Youle, R. J. The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy. Nature 524: 309-314, 2015. 3. Thurston, T. L. M., Wandel, M. P., von Muhlinen, N., Foeglein, A., Randow, F. Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion. Nature 482: 414-418, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CALCOCO2

Target: CALCOCO2 (Calcium Binding and Coiled-Coil Domain 2 (CALCOCO2))

Alternative Name: CALCOCO2

Background:

Synonyms: Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, GOLGA2

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 52 kDa

Gene ID: 10241

UniProt: Q13137