SHMT1 antibody (AA 1-470)

Catalog No. ABIN7599420

This anti-SHMT1 antibody is a Mouse Monoclonal antibody detecting SHMT1 in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for SHMT1 antibody (AA 1-470) (ABIN7599420)

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 9C7

Product Details anti-SHMT1 Antibody

Binding Specificity: AA 1-470

Purpose: Anti-SHMT1 Antibody Picoband® (monoclonal, 9C7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SHMT1 Antibody Picoband® (monoclonal, 9C7) (ABIN7599420). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SHMT1 recombinant protein (Position: M1-S470).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Byrne, P. C., Shipley, J. M., Chave, K. J., Sanders, P. G., Snell, K. Characterisation of a human serine hydroxymethyltransferase pseudogene and its localisation of 1p32.3-33. Hum. Genet. 97: 340-344, 1996. 2. Elsea, S. H., Juyal, R. C., Jiralerspong, S., Finucane, B. M., Pandolfo, M., Greenberg, F., Baldini, A., Stover, P., Patel, P. I. Haploinsufficiency of cytosolic serine hydroxymethyltransferase in the Smith-Magenis syndrome. Am. J. Hum. Genet. 57: 1342-1350, 1995. 3. Garrow, T. A., Brenner, A. A., Whitehead, V. M., Chen, X.-N., Duncan, R. G., Korenberg, J. R., Shane, B. Cloning of human cDNAs encoding mitochondrial and cytosolic serine hydroxymethyltransferases and chromosomal localization. J. Biol. Chem. 268: 11910-11916, 1993.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SHMT1

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Alternative Name: SHMT1

Background:

Synonyms: "chromosomal protein, Nonhistone, HMG4 antibody|High mobility group (nonhistone chromosomal) protein 4 antibody|High mobility group box 3 antibody|High mobility group protein 2a antibody|High mobility group protein 4 antibody|High mobility group protein B3 antibody| High mobility group protein HMG4 antibody|HMG 4 antibody|HMG-2a antibody|HMG-4 antibody|HMG2A antibody|HMGB 3 antibody|HMGB3 antibody| HMGB3_HUMAN antibody|MGC90319 antibody|Non histone chromosomal protein antibody|Nonhistone chromosomal protein HMG4 antibody"

Tissue Specificity: Expressed predominantly in placenta.

Background: This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.

Molecular Weight: 53 kDa

Gene ID: 6470

UniProt: P34896