IKZF2 antibody (AA 1-477)

Catalog No. ABIN7599427

This Rabbit Polyclonal antibody specifically detects IKZF2 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IKZF2 antibody (AA 1-477) (ABIN7599427)

Target: IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKZF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-IKZF2 Antibody

Binding Specificity: AA 1-477

Purpose: Anti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ZNFN1A2/HELIOS/IKZF2 Antibody Picoband® (ABIN7599427). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ZNFN1A2/HELIOS/IKZF2 recombinant protein (Position: M1-R477).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chessum, L., Matern, M. S., Kelly, M. C., Johnson, S. L., Ogawa, Y., Milon, B., McMurray, M., Driver, E. C., Parker, A., Song, Y., Codner, G., Esapa, C. T., and 11 others. Helios is a key transcriptional regulator of outer hair cell maturation. Nature 563: 696-700, 2018. 2. Gross, M. B. Personal Communication. Baltimore, Md. 2/15/2012. 3. Hosokawa, Y., Maeda, Y., Seto, M. Human Helios, an Ikaros-related zinc finger DNA binding protein: cDNA cloning and tissue expression pattern. Immunogenetics 50: 106-108, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IKZF2

Target: IKZF2 (IKAROS Family Zinc Finger 2 (IKZF2))

Alternative Name: IKZF2

Background:

Synonyms: Zinc finger protein Helios, Ikaros family zinc finger protein 2, IKZF2, HELIOS, ZNFN1A2

Tissue Specificity: Ubiquitous. High expression in liver, spleen, pancreas and kidney.

Background: Zinc finger protein Helios is a protein that in humans is encoded by the IKZF2 gene. This gene is mapped to 2q34.This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Molecular Weight: 70 kDa

Gene ID: 22807