PTBP2 antibody (AA 1-504)

Catalog No. ABIN7599452

The Rabbit Polyclonal anti-PTBP2 antibody has been validated for WB, ELISA, IHC, IF, ICC and FACS. It is suitable to detect PTBP2 in samples from Human, Mouse and Rat.

Quick Overview for PTBP2 antibody (AA 1-504) (ABIN7599452)

Target: PTBP2 (Polypyrimidine Tract Binding Protein 2 (PTBP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTBP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PTBP2 Antibody

Binding Specificity: AA 1-504

Purpose: Anti-PTBP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PTBP2 Antibody Picoband® (ABIN7599452). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PTBP2 recombinant protein (Position: M1-A504).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Makeyev, E. V., Zhang, J., Carrasco, M. A., Maniatis, T. The microRNA miR-124 promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing. Molec. Cell 27: 435-448, 2007. 2. Rahman, L., Bliskovski, V., Kaye, F. J., Zajac-Kaye, M. Evolutionary conservation of a 2-kb intronic sequence flanking a tissue-specific alternative exon in the PTBP2 gene. Genomics 83: 76-84, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PTBP2

Target: PTBP2 (Polypyrimidine Tract Binding Protein 2 (PTBP2))

Alternative Name: PTBP2

Background:

Synonyms: Polypyrimidine tract-binding protein 2, Neural polypyrimidine tract-binding protein, Neurally-enriched homolog of PTB, PTB-like protein, PTBP2, NPTB, PTB, PTBLP

Tissue Specificity: Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform 6 are truncated forms expressed in non-neuronal tissues.

Background: Polypyrimidine tract binding protein 2, also known as PTBP2, is a protein which in humans is encoded by the PTBP2 gene. It is mapped to 1p21.3. The protein encoded by this gene binds to intronic polypyrimidine clusters in pre-mRNA molecules and is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein (PTB) but most of its isoforms are expressed primarily in the brain. Alternative splicing results in multiple transcript variants.

Molecular Weight: 57 kDa

Gene ID: 58155

Pathways: Ribonucleoprotein Complex Subunit Organization, SARS-CoV-2 Protein Interactome