NCF2 antibody (AA 1-526)

Catalog No. ABIN7599462

This anti-NCF2 antibody is a Rabbit Polyclonal antibody detecting NCF2 in WB, IHC, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NCF2 antibody (AA 1-526) (ABIN7599462)

Target: NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-NCF2 Antibody

Binding Specificity: AA 1-526

Purpose: Anti-NOXA2/p67phox/NCF2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOXA2/p67phox/NCF2 Antibody Picoband® (ABIN7599462). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOXA2/p67phox/NCF2 recombinant protein (Position: M1-V526).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Francke, U., Hsieh, C.-L., Foellmer, B. E., Lomax, K. J., Malech, H. L., Leto, T. L. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am. J. Hum. Genet. 47: 483-492, 1990. 2. Noack, D., Rae, J., Cross, A. R., Munoz, J., Salmen, S., Mendoza, J. A., Rossi, N., Curnutte, J. T., Heyworth, P. G. Autosomal recessive chronic granulomatous disease caused by novel mutations in NCF-2, the gene encoding the p67-phox component of phagocyte NADPH oxidase. Hum. Genet. 105: 460-467, 1999. 3. Sancho-Shimizu, V., Malo, D. Sequencing, expression, and functional analyses support the candidacy of Ncf2 in susceptibility to Salmonella Typhimurium infection in wild-derived mice. J. Immun. 176: 6954-6961, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NCF2

Target: NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))

Alternative Name: NCF2

Background:

Synonyms: Syntenin-1, Melanoma differentiation-associated protein 9, MDA-9, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, TACIP18, Scaffold protein Pbp1, Syndecan-binding protein 1, SDCBP, MDA9, SYCL

Tissue Specificity: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

Background: This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 60 kDa

Gene ID: 4688

UniProt: P19878