REC8 antibody (AA 1-547)

Catalog No. ABIN7599473

The Rabbit Polyclonal anti-REC8 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect REC8 in samples from Human and Rat.

Quick Overview for REC8 antibody (AA 1-547) (ABIN7599473)

Target: REC8 (REC8 Homolog (Yeast) (REC8))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REC8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-REC8 Antibody

Binding Specificity: AA 1-547

Purpose: Anti-REC8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-REC8 Antibody Picoband® (ABIN7599473). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human REC8 recombinant protein (Position: M1-H547).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brar, G. A., Kiburz, B. M., Zhang, Y., Kim, J.-E., White, F., Amon, A. Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis. Nature 441: 532-536, 2006. 2. Parisi, S., McKay, M. J., Molnar, M., Thompson, M. A., van der Spek, P. J., van Drunen-Schoenmaker, E., Kanaar, R., Lehmann, E., Hoeijmakers, J. H. J., Kohli, J. Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Molec. Cell. Biol. 19: 3515-3528, 1999. 3. Xu, H., Beasley, M. D., Warren, W. D., van der Horst, G. T. J., McKay, M. J. Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis. Dev. Cell 8: 949-961, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for REC8

Target: REC8 (REC8 Homolog (Yeast) (REC8))

Alternative Name: REC8

Background:

Synonyms: Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1

Tissue Specificity: Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa.

Background: Meiotic recombination protein REC8 homolog is a protein that in humans is encoded by the REC8 gene. It is mapped to 14q12. This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

Molecular Weight: 63 kDa

Gene ID: 9985

UniProt: O95072