RUFY4 antibody (AA 1-553)

Catalog No. ABIN7599475

Product Details anti-RUFY4 Antibody

Target: RUFY4 (RUN and FYVE Domain Containing 4 (RUFY4))

Binding Specificity: AA 1-553

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUFY4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Anti-RUFY4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RUFY4 Antibody Picoband® (ABIN7599475). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RUFY4 recombinant protein (Position: M1-K553).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Terawaki, S. , Camosseto, V. , Prete, F. , Wenger, T. , Papadopoulos, A. , & Rondeau, C. , et al. (2015). Run and fyve domain-containing protein 4 enhances autophagy and lysosome tethering in response to interleukin-4. The Journal of Cell Biology, 210(7), 1133-1152. 2. Chen, S. , Robert, F. , Lambertus, K. , Yuan, L. , Wang, J. , & Kathryn, R. , et al. (2018). An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders. Nature Genetics. 3. Haenig, C. , Atias, N. , Taylor, A. K. , Mazza, A. , & Wanker, E. E. . (2020). Interactome mapping provides a network of neurodegenerative disease proteins and uncovers widespread protein aggregation in affected brains. Cell Reports, 32(7), 108050.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RUFY4

Target: RUFY4 (RUN and FYVE Domain Containing 4 (RUFY4))

Alternative Name: RUFY4 (RUFY4 Products)

Background:

Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: Enables phosphatidylinositol-3-phosphate binding activity. Involved in autophagosome assembly, cellular response to interleukin-4, and positive regulation of macroautophagy. Located in autophagosome.

Molecular Weight: 64 kDa

Gene ID: 285180