GRHL1 antibody (AA 1-618)

Catalog No. ABIN7599498

This anti-GRHL1 antibody is a Rabbit Polyclonal antibody detecting GRHL1 in WB, ELISA, ICC, IF and FACS. Suitable for Human.

Quick Overview for GRHL1 antibody (AA 1-618) (ABIN7599498)

Target: GRHL1 (Grainyhead-Like 1 (GRHL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRHL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GRHL1 Antibody

Binding Specificity: AA 1-618

Purpose: Anti-Grainyhead-like protein 1/GRHL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Grainyhead-like protein 1/GRHL1 Antibody Picoband® (ABIN7599498). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Grainyhead-like protein 1/GRHL1 recombinant protein (Position: M1-I618).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Huang, N., Miller, W. L. Cloning of factors related to HIV-inducible LBP proteins that regulate steroidogenic factor-1-independent human placental transcription of the cholesterol side-chain cleavage enzyme, P450scc. J. Biol. Chem. 275: 2852-2858, 2000. 2. Jacobs, J., Atkins, M., Davie, K., Imrichova, H., Romanelli, L., Christiaens, V., Hulselmans, G., Potier, D., Wouters, J., Taskiran, I. I., Paciello, G., Gonzalez-Blas, C. B., Koldere, D., Aibar, S., Halder, G., Aerts, S. The transcription factor grainy head primes epithelial enhancers for spatiotemporal activation by displacing nucleosomes. Nature Genet. 50: 1011-1020, 2018.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GRHL1

Target: GRHL1 (Grainyhead-Like 1 (GRHL1))

Alternative Name: GRHL1

Background:

Synonyms: Troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, TNNI2

Tissue Specificity: Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .

Background: This gene encodes a member of the grainyhead family of transcription factors. The encoded protein can exist as a homodimer or can form heterodimers with sister-of-mammalian grainyhead or brother-of-mammalian grainyhead. This protein functions as a transcription factor during development.

Molecular Weight: 70 kDa

Gene ID: 29841

Pathways: Regulation of Lipid Metabolism by PPARalpha