Calpain 6 antibody (AA 1-641)

Catalog No. ABIN7599511

This Rabbit Polyclonal antibody specifically detects Calpain 6 in WB, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Calpain 6 antibody (AA 1-641) (ABIN7599511)

Target: Calpain 6 (CAPN6)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Calpain 6 Antibody

Binding Specificity: AA 1-641

Purpose: Anti-Calpain 6/CAPN6 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Calpain 6-CAPN6 Antibody Picoband® (ABIN7599511). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Calpain 6/CAPN6 recombinant protein (Position: M1-L641). Human Calpain 6/CAPN6 shares 95.2% and 95% amino acid (aa) sequence identity with mouse and rat Calpain 6/CAPN6, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL
1. Dear, N., Matena, K., Vingron, M., Boehm, T. A new subfamily of vertebrate calpains lacking a calmodulin-like domain: implications for calpain regulation and evolution. Genomics 45: 175-184, 1997. 2. Matena, K., Boehm, T., Dear, T. N. Genomic organization of mouse Capn5 and Capn6 genes confirms that they are a distinct calpain subfamily. Genomics 48: 117-120, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Calpain 6

Target: Calpain 6 (CAPN6)

Alternative Name: CAPN6

Background:

Synonyms: CAPN6, CALPM, CANPX, Calpain-6, Calpain-like protease X-linked, Calpamodulin, CalpM

Background: Calpain-6 (also known as calpamodulin) is a protein in humans that is encoded by the CAPN6 gene. Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis.

Molecular Weight: 75 kDa

Gene ID: 827

UniProt: Q9Y6Q1