PDE4B antibody (AA 1-736)

Catalog No. ABIN7599537

This anti-PDE4B antibody is a Rabbit Polyclonal antibody detecting PDE4B in WB and ELISA. Suitable for Human and Mouse.

Quick Overview for PDE4B antibody (AA 1-736) (ABIN7599537)

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE4B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PDE4B Antibody

Binding Specificity: AA 1-736

Purpose: Anti-PDE4B Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PDE4B Antibody Picoband® (ABIN7599537). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDE4B recombinant protein (Position: M1-T736). Human PDE4B shares 96.6% amino acid (aa) sequence identity with rat PDE4B.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
ELISA, 0.1-0.5 μg/mL
1. Bolger, G., Michaeli, T., Martins, T., St. John, T., Steiner, B., Rodgers, L., Riggs, M., Wigler, M., Ferguson, K. A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs. Molec. Cell. Biol. 13: 6558-6571, 1993. 2. Huston, E., Lumb, S., Russell, A., Catterall, C., Ross, A. H., Steele, M. R., Bolger, G. B., Perry, M. J., Owens, R. J., Houslay, M. D. Molecular cloning and transient expression in COS7 cells of a novel human PDE4B cAMP-specific phosphodiesterase, HSPDE4B3. Biochem. J. 328: 549-558, 1997. 3. Livi, G. P., Kmetz, P., McHale, M. M., Cieslinski, L. B., Sathe, G. M., Taylor, D. P., Davis, R. L., Torphy, T. J., Balcarek, J. M. Cloning and expression of cDNA for a human low-K(m), rolipram-sensitive cyclic AMP phosphodiesterase. Molec. Cell. Biol. 10: 2678-2686, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDE4B

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B

Background:

Synonyms: PDE4B, DPDE4, cAMP-specific 3',5'-cyclic phosphodiesterase 4B, EC 3.1.4.53, DPDE4, PDE32

Background: This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms.

Molecular Weight: 83 kDa

Gene ID: 5142

UniProt: Q07343

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction