Axin antibody (AA 1-811)

Catalog No. ABIN7599556

This Rabbit Polyclonal antibody specifically detects Axin in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Axin antibody (AA 1-811) (ABIN7599556)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Axin Antibody

Binding Specificity: AA 1-811

Purpose: Anti-AXIN1 Antibody Picoband®

Characteristics: Anti-AXIN1 Antibody Picoband® (ABIN7599556). Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AXIN1 recombinant protein (Position: M1-K811). Human AXIN1 shares 86.2% and 82.8% amino acid (aa) sequence identity with mouse and rat AXIN1, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, H.-J., Lin, C.-M., Lin, C.-S., Perez-Olle, R., Leung, C. L., Liem, R. K. H. The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway. Genes Dev. 20: 1933-1945, 2006. 2. Goto, T., Matsuzawa, J., Iemura, S., Natsume, T., Shibuya, H. WDR26 is a new partner of Axin1 in the canonical Wnt signaling pathway. FEBS Lett. 590: 1291-1303, 2016. 3. Huang, S.-M. A., Mishina, Y. M., Liu, S., Cheung, A., Stegmeier, F., Michaud, G. A., Charlat, O., Wiellette, E., Zhang, Y., Wiessner, S., Hild, M., Shi, X., and 24 others. Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461: 614-620, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: AXIN1

Background: Axin-1 is a protein that in humans is encoded by the AXIN1 gene. This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Molecular Weight: 110 kDa

Gene ID: 8312

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling