NDFIP1 antibody (AA 10-221)

Catalog No. ABIN7599591

This anti-NDFIP1 antibody is a Rabbit Polyclonal antibody detecting NDFIP1 in ELISA, WB and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for NDFIP1 antibody (AA 10-221) (ABIN7599591)

Target: NDFIP1 (Nedd4 Family Interacting Protein 1 (NDFIP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDFIP1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-NDFIP1 Antibody

Binding Specificity: AA 10-221

Purpose: Anti-NDFIP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NDFIP1 Antibody Picoband® (ABIN7599591). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NDFIP1 recombinant protein (Position: A10-Y221).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Foot, N. J., Leong, Y. A., Dorstyn, L. E., Dalton, H. E., Ho, K., Zhao, L., Garrick, M. D., Yang, B., Hiwase, D., Kumar, S. Ndfip1-deficient mice have impaired DMT1 regulation and iron homeostasis. Blood 117: 638-646, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/11/2008. 3. Harvey, K. F., Shearwin-Whyatt, L. M., Fotia, A., Parton, R. G., Kumar, S. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 277: 9307-9317, 2002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NDFIP1

Target: NDFIP1 (Nedd4 Family Interacting Protein 1 (NDFIP1))

Alternative Name: NDFIP1

Background:

Synonyms: Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, hKCC2, Neuronal K-Cl cotransporter, SLC12A5, KCC2, KIAA1176

Tissue Specificity: Brain specific. Detected in neuronal cells.

Background: Nedd4 family interacting protein 1 is a protein that in humans is encoded by the NDFIP1 gene. The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins.

Molecular Weight: 28 kDa

Gene ID: 80762

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity