PYCR2 antibody (AA 10-320)

Catalog No. ABIN7599597

This anti-PYCR2 antibody is a Rabbit Polyclonal antibody detecting PYCR2 in WB, ELISA, IF, FACS and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for PYCR2 antibody (AA 10-320) (ABIN7599597)

Target: PYCR2 (Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYCR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PYCR2 Antibody

Binding Specificity: AA 10-320

Purpose: Anti-PYCR2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PYCR2 Antibody Picoband® (ABIN7599597). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PYCR2 recombinant protein (Position: Q10-D320).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. De Ingeniis, J., Ratnikov, B., Richardson, A. D., Scott, D. A., Aza-Blanc, P., De, S. K., Kazanov, M., Pellecchia, M., Ronai, Z., Osterman, A. L., Smith, J. W. Functional specialization in proline biosynthesis of melanoma. PLoS One 7: e45190, 2012. Note: Electronic Article. 2. Escande-Beillard, N., Loh, A., Saleem, S. N., Kanata, K., Hashimoto, Y., Altunoglu, U., Metoska, A., Grandjean, J., Ng, F. M., Pomp, O., Baburajendran, N., Wong, J., Hill, J., Beillard, E., Cozzone, P., Zaki, M., Kayserili, H., Hamada, H., Shiratori, H., Reversade, B. Loss of PYCR2 causes neurodegeneration by increasing cerebral glycine levels via SHMT2. Neuron 107: 82-94, 2020. 3. Nakayama, T., Al-Maawali, A., El-Quessny, M., Rajab, A., Khalil, S., Stoler, J. M., Tan, W.-H., Nasir, R., Schmitz-Abe, K., Hill, R. S., Partlow, J. N., Al-Saffar, M., and 10 others. Mutations in PYCR2, encoding pyrroline-5-carboxylate reductase 2, cause microcephaly and hypomyelination. Am. J. Hum. Genet. 96: 709-719, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PYCR2

Target: PYCR2 (Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2))

Alternative Name: PYCR2

Background:

Synonyms: Pre T-cell antigen receptor alpha, pT-alpha, pTa, pT-alpha-TCR, PTCRA

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Pyrroline-5-carboxylate reductase family, member 2 is a protein that in humans is encoded by the PYCR2 gene. This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene.

Molecular Weight: 35 kDa

Gene ID: 29920

UniProt: Q96C36