Phospholipase C delta 3 antibody (AA 10-702)

Catalog No. ABIN7599611

The Rabbit Polyclonal anti-Phospholipase C delta 3 antibody has been validated for WB, ELISA, ICC, FACS and IF. It is suitable to detect Phospholipase C delta 3 in samples from Human.

Quick Overview for Phospholipase C delta 3 antibody (AA 10-702) (ABIN7599611)

Target: Phospholipase C delta 3 (PLCd3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C delta 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Phospholipase C delta 3 Antibody

Binding Specificity: AA 10-702

Purpose: Anti-PLCD3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PLCD3 Antibody Picoband® (ABIN7599611). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLCD3 recombinant protein (Position: R10-Q702).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ghosh, S., Pawelczyk, T., Lowenstein, J. M. Phospholipase C isoforms delta-1 and delta-3 from human fibroblasts: high-yield expression in Escherichia coli, simple purification, and properties. Protein Expr. Purif. 9: 262-278, 1997. 2. Kim, H., Suh, P. G., Ryu, S. H., Park, S. H. Assignment of the human PLC-delta-3 gene (PLCD3) to human chromosome band 17q21 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 87: 209-210, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Phospholipase C delta 3

Target: Phospholipase C delta 3 (PLCd3)

Alternative Name: PLCD3

Background:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3 is an enzyme that in humans is encoded by the PLCD3 gene. This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids.

Molecular Weight: 94 kDa

Gene ID: 113026

UniProt: Q8N3E9

Pathways: WNT Signaling