Amphiregulin antibody (AA 101-180)

Catalog No. ABIN7599624

This anti-Amphiregulin antibody is a Rabbit Polyclonal antibody detecting Amphiregulin in WB, ELISA and FACS. Suitable for Human.

Quick Overview for Amphiregulin antibody (AA 101-180) (ABIN7599624)

Target: Amphiregulin (AREG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amphiregulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Amphiregulin Antibody

Binding Specificity: AA 101-180

Purpose: Anti-Amphiregulin/AREG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Amphiregulin/AREG Antibody Picoband® (ABIN7599624). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Amphiregulin/AREG recombinant protein (Position: S101-R180).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Ciarloni, L., Mallepell, S., Brisken, C. Amphiregulin is an essential mediator of estrogen receptor alpha function in mammary gland development. Proc. Nat. Acad. Sci. 104: 5455-5460, 2007. 2. Cook, P. W., Piepkorn, M., Clegg, C. H., Plowman, G. D., DeMay, J. M., Brown, J. R., Pittelkow, M. R. Transgenic expression of the human amphiregulin gene induces a psoriasis-like phenotype. J. Clin. Invest. 100: 2286-2294, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Amphiregulin

Target: Amphiregulin (AREG)

Alternative Name: AREG

Background:

Synonyms: Amphiregulin, AR, Schwannoma-derived growth factor, SDGF, Areg, Sdgf

Tissue Specificity: Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.

Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor (EGF) family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor (EGFR) to promote the growth of normal epithelial cells. It is mapped to 9q32. It has been shown to play a role in immunity, inflammation, tissue repair, and lung and mammary gland development. Homozygous knockout mice for this gene exhibit impaired immune system regulation in the skin and gene expression changes characteristic of chronic liver damage.

Molecular Weight: 45 kDa

Gene ID: 374

UniProt: P15514

Pathways: RTK Signaling, EGFR Signaling Pathway