YTHDC2 antibody (AA 105-1231)

Catalog No. ABIN7599661

Product Details anti-YTHDC2 Antibody

Target: YTHDC2 (YTH Domain Containing 2 (YTHDC2))

Binding Specificity: AA 105-1231

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YTHDC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: Anti-YTHDC2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-YTHDC2 Antibody Picoband® (ABIN7599661). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human YTHDC2 recombinant protein (Position: K105-K1231).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fanale, D., Iovanna, J. L., Calvo, E. L., Berthezene, P., Belleau, P., Dagorn, J. C., Bronte, G., Cicero, G., Bazan, V., Rolfo, C., Santini, D., Russo, A. Germline copy number variation in the YTHDC2 gene: does it have a role in finding a novel potential molecular target involved in pancreatic adenocarcinoma susceptibility? Expert Opin. Ther. Targets 18: 841-850, 2014. 2. Jain, D., Puno, M. R., Meydan, C., Lailler, N., Mason, C. E., Lima, C. D., Anderson, K. V., Keeney, S. ketu mutant mice uncover an essential meiotic function for the ancient RNA helicase YTHDC2. eLife 7: e30919, 2018. Note: Electronic Article. 3. Morohashi, K., Sahara, H., Watashi, K., Iwabata, K., Sunoki, T., Kuramochi, K., Takakusagi, K., Miyashita, H., Sato, N., Tanabe, A., Shimotohno, K., Kobayashi, S., Sakaguchi, K., Sugawara, F. Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B. PLoS One 6: e18285, 2011. Note: Electronic Article.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for YTHDC2

Target: YTHDC2 (YTH Domain Containing 2 (YTHDC2))

Alternative Name: YTHDC2 (YTHDC2 Products)

Background:

Synonyms: One cut domain family member 2, Hepatocyte nuclear factor 6-beta, HNF-6-beta, One cut homeobox 2, Transcription factor ONECUT-2, OC-2, ONECUT2, HNF6B

Tissue Specificity: Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Background: YTH domain containing 2 is a protein that in humans is encoded by the YTHDC2 gene. This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line.

Molecular Weight: 175 kDa

Gene ID: 64848

UniProt: Q9H6S0