LIMS1 antibody (AA 11-172)

Catalog No. ABIN7599708

This anti-LIMS1 antibody is a Rabbit Polyclonal antibody detecting LIMS1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for LIMS1 antibody (AA 11-172) (ABIN7599708)

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LIMS1 Antibody

Binding Specificity: AA 11-172

Purpose: Anti-LIMS1 Antibody Picoband®

Characteristics: Anti-LIMS1 Antibody Picoband® (ABIN7599708). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LIMS1 recombinant protein (Position: E11-A172). Human LIMS1 shares 100% amino acid (aa) sequence identity with mouse LIMS1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Bock-Marquette, I., Saxena, A., White, M. D., DiMaio, J. M., Srivastava, D. Thymosin beta-4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature 432: 466-472, 2004. 2. Chen, K., Tu, Y., Zhang, Z., Blair, H. C., Zhang, L., Wu, C. PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells. J. Biol. Chem. 283: 2508-2517, 2008. 3. Fukuda, T., Chen, K., Shi, X., Wu, C. PINCH-1 is an obligate partner of integrin-linked kinase (ILK) functioning in cell shape modulation, motility, and survival. J. Biol. Chem. 278: 51324-51333, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LIMS1

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Alternative Name: LIMS1

Background: LIM and senescent cell antigen-like-containing domain protein 1 is a protein that in humans is encoded by the LIMS1 gene. The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 37 kDa

Gene ID: 3987

UniProt: P48059