LIMS1 antibody

Catalog No. ABIN969354

Cited in 2+ publications. The Mouse Monoclonal anti-LIMS1 antibody (Clone 5G7) (ABIN969354) specifically detects LIMS1 in ELISA, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for LIMS1 antibody (ABIN969354)

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIMS1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 5G7

Product Details anti-LIMS1 Antibody

Purpose: PINCH Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human PINCH expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-LIMS1 antibody (ABIN969354)

Yang, Wang, Hawkins, Chen, Vaynberg, Mao, Tu, Zuo, Wang, Wang, Wu, Tjandra, Qin: "Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase." in: The Journal of biological chemistry, Vol. 284, Issue 9, pp. 5836-44, (2009) (PubMed).

Chiswell, Zhang, Murphy, Boggon, Calderwood: "The structural basis of integrin-linked kinase-PINCH interactions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 52, pp. 20677-82, (2008) (PubMed).

Target Details for LIMS1

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Alternative Name: PINCH

Background: The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 37 kDa

Gene ID: 3987

UniProt: P48059