PDE6A antibody (AA 11-237)

Catalog No. ABIN7599710

Product Details anti-PDE6A Antibody

Target: PDE6A (phosphodiesterase 6A, CGMP-Specific, Rod, alpha (PDE6A))

Binding Specificity: AA 11-237

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDE6A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-PDE6 alpha/PDE6A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDE6 alpha/PDE6A Antibody Picoband® (ABIN7599710). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDE6 alpha/PDE6A recombinant protein (Position: K11-R237).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Corton, M., Blanco, M. J., Torres, M., Sanchez-Salorio, M., Carracedo, A., Brion, M. Identification of a novel mutation in the human PDE6A gene in autosomal recessive retinitis pigmentosa: homology with the nmf28/nmf28 mice model. (Letter) Clin. Genet. 78: 495-498, 2010. 2. Khan, S. Y., Ali, S., Naeem, M. A., Khan, S. N., Husnain, T., Butt, N. H., Qazi, Z. A., Akram, J., Riazuddin, S., Ayyagari, R., Hejtmancik, J. F., Riazuddin, S. A. Splice-site mutations identified in PDE6A responsible for retinitis pigmentosa in consanguineous Pakistani families. Molec. Vision 21: 871-882, 2015. 3. Tuntivanich, N., Pittler, S. J., Fischer, A. J., Omar, G., Kiupel, M., Weber, A., Yao, S., Steibel, J. P., Khan, N. W., Petersen-Jones, S. M. Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation. Invest. Ophthal. Vis. Sci. 50: 801-813, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PDE6A

Target: PDE6A (phosphodiesterase 6A, CGMP-Specific, Rod, alpha (PDE6A))

Alternative Name: PDE6A (PDE6A Products)

Background:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.

Molecular Weight: 100 kDa

Gene ID: 5145

UniProt: P16499

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction