DPH5 antibody (AA 11-250)

Catalog No. ABIN7599711

This anti-DPH5 antibody is a Rabbit Polyclonal antibody detecting DPH5 in WB, ELISA, IF, FACS and ICC. Suitable for Human.

Quick Overview for DPH5 antibody (AA 11-250) (ABIN7599711)

Target: DPH5 (DPH5 Homolog (DPH5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-DPH5 Antibody

Binding Specificity: AA 11-250

Purpose: Anti-DPH5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DPH5 Antibody (ABIN7599711). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DPH5 recombinant protein (Position: D11-H250).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DPH5

Target: DPH5 (DPH5 Homolog (DPH5))

Alternative Name: DPH5

Background:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 6, Glucose transporter type 6, GLUT-6, Glucose transporter type 9, GLUT-9, SLC2A6, GLUT9

Tissue Specificity: Highly expressed in brain, spleen and peripheral blood leukocytes.

Background: Diphthine synthase is an enzyme that in humans is encoded by the DPH5 gene. This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 37 kDa

Gene ID: 51611

UniProt: Q9H2P9

Pathways: SARS-CoV-2 Protein Interactome