ATF5 antibody (AA 11-275)

Catalog No. ABIN7599712

This Rabbit Polyclonal antibody specifically detects ATF5 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATF5 antibody (AA 11-275) (ABIN7599712)

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ATF5 Antibody

Binding Specificity: AA 11-275

Purpose: Anti-ATF5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ATF5 Antibody Picoband® (ABIN7599712). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATF5 recombinant protein (Position: L11-R275). Human ATF5 shares 86.5% and 87.2% amino acid (aa) sequence identity with mouse and rat ATF5, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Pati, D., Meistrich, M. L., Plon, S. E. Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis. Molec. Cell. Biol. 19: 5001-5013, 1999. 2. Peters, C. S., Liang, X., Li, S., Kannan, S., Peng, Y., Taub, R., Diamond, R. H. ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase. J. Biol. Chem. 276: 13718-13726, 2001. 3. Sheng, Z., Li, L., Zhu, L. J., Smith, T. W., Demers, A., Ross, A. H., Moser, R. P., Green, M. R. A genome-wide RNA interference screen reveals an essential CREB3L2-ATF5-MCL1 survival pathway in malignant glioma with therapeutic implications. Nature Med. 16: 671-677, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATF5

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Alternative Name: ATF5

Background:

Synonyms: ATF5, ATFX, Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx

Background: Activating transcription factor 5, also known as ATF5, is a protein that, in humans, is encoded by the ATF5 gene. Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific, RNA polymerase II transcription regulatory region sequence-specific DNA binding activity, and tubulin binding activity. Involved in several processes, including fat cell differentiation, regulation of cell cycle process, and regulation of transcription, DNA-templated. Located in centrosome, cytosol, and nucleoplasm.

Molecular Weight: 37 kDa

Gene ID: 22809

UniProt: Q9Y2D1

Pathways: Myometrial Relaxation and Contraction