PET112L antibody (AA 113-443)

Catalog No. ABIN7599754

This Rabbit Polyclonal antibody specifically detects PET112L in WB, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for PET112L antibody (AA 113-443) (ABIN7599754)

Target: PET112L (PET112) (PET112 Homolog (PET112))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PET112L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PET112L Antibody

Binding Specificity: AA 113-443

Purpose: Anti-GATB Antibody Picoband®

Characteristics: Anti-GATB Antibody Picoband® (ABIN7599754). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GATB recombinant protein (Position: R113-E443). Human GATB shares 89.7% amino acid (aa) sequence identity with mouse GATB.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Friederich, M. W., Timal, S., Powell, C. A., Dallabona, C., Kurolap, A., Palacios-Zambrano, S., Bratkovic, D., Derks, T. G. J., Bick, D., Bouman, K., Chatfield, K. C., Damouny-Naoum, N., and 41 others. Pathogenic variants in glutamyl-tRNA(Gln) amidotransferase subunits cause a lethal mitochondrial cardiomyopathy disorder. Nature Commun. 9: 4065, 2018. Note: Electronic Article. 2. Gross, M. B. Personal Communication. Baltimore, Md. 11/30/2016. 3. Nagao, A., Suzuki, T., Katoh, T., Sakaguchi, Y., Suzuki, T. Biogenesis of glutaminyl-mt tRNA(Gln) in human mitochondria. Proc. Nat. Acad. Sci. 106: 16209-16214, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PET112L

Target: PET112L (PET112) (PET112 Homolog (PET112))

Alternative Name: GATB

Background: GATB allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln).

Molecular Weight: 62 kDa

Gene ID: 5188

UniProt: O75879