ZIC3 antibody (AA 113-455)

Catalog No. ABIN7599755

The Rabbit Polyclonal anti-ZIC3 antibody has been validated for WB, ELISA and FACS. It is suitable to detect ZIC3 in samples from Human, Mouse and Rat.

Quick Overview for ZIC3 antibody (AA 113-455) (ABIN7599755)

Target: ZIC3 (Zic Family Member 3 (ZIC3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZIC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ZIC3 Antibody

Binding Specificity: AA 113-455

Purpose: Anti-ZIC3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ZIC3 Antibody Picoband® (ABIN7599755). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ZIC3 recombinant protein (Position: N113-N455).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aruga, J., Nagai, T., Tokuyama, T., Hayashizaki, Y., Okazaki, Y., Chapman, V. M., Mikoshiba, K. The mouse Zic gene family: homologues of the Drosophila pair-rule gene odd-paired. J. Biol. Chem. 271: 1043-1047, 1996. 2. Bedard, J. E. J., Purnell, J. D., Ware, S. M. Nuclear import and export signals are essential for proper cellular trafficking and function of ZIC3. Hum. Molec. Genet. 16: 187-198, 2007. 3. Burdine, R. D., Schier, A. F. Conserved and divergent mechanisms in left-right axis formation. Genes Dev. 14: 763-776, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZIC3

Target: ZIC3 (Zic Family Member 3 (ZIC3))

Alternative Name: ZIC3

Background:

Synonyms: Microtubule-associated tumor suppressor 1, AT2 receptor-binding protein, Angiotensin-II type 2 receptor-interacting protein, Mitochondrial tumor suppressor 1, MTUS1, ATBP, ATIP, GK1, KIAA1288, MTSG1

Tissue Specificity: Ubiquitously expressed (at protein level). Highly expressed in brain. Down-regulated in ovarian carcinoma, pancreas carcinoma, colon carcinoma and head and neck squamous cell carcinoma (HNSCC). Isoform 1 is the major isoform in most peripheral tissues. Isoform 2 is abundant in most peripheral tissues. Isoform 3 is the major isoform in brain, female reproductive tissues, thyroid and heart. Within brain it is highly expressed in corpus callosum and pons. Isoform 6 is brain-specific, it is the major isoform in cerebellum and fetal brain.

Background: ZIC3 is a member of the Zinc finger of the cerebellum (ZIC) protein family. This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.

Molecular Weight: 56-60 kDa

Gene ID: 7547

UniProt: O60481