ILKAP antibody (AA 114-436)

Catalog No. ABIN7599758

The Rabbit Polyclonal anti-ILKAP antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect ILKAP in samples from Human.

Quick Overview for ILKAP antibody (AA 114-436) (ABIN7599758)

Target: ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILKAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ILKAP Antibody

Binding Specificity: AA 114-436

Purpose: Anti-ILKAP Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-ILKAP Antibody Picoband® (ABIN7599758). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDK1 recombinant protein (Position: Y114-A436). Human PDK1 shares 94.7% and 95.7% amino acid (aa) sequence identity with mouse and rat PDK1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 6/10/2020. 2. Kumar, A. S., Naruszewicz, I., Wang, P., Leung-Hagesteijn, C., Hannigan, G. E. ILKAP regulates ILK signaling and inhibits anchorage-independent growth. Oncogene 23: 3454-3461, 2004. 3. Leung-Hagesteijn, C., Mahendra, A., Naruszewicz, I., Hannigan, G. E. Modulation of integrin signal transduction by ILKAP, a protein phosphatase 2C associating with the integrin-linked kinase, ILK1. EMBO J. 20: 2160-2170, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ILKAP

Target: ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))

Alternative Name: ILKAP

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Integrin-linked kinase-associated serine/threonine phosphatase 2C is an enzyme that in humans is encoded by the ILKAP gene. The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Molecular Weight: 43 kDa

Gene ID: 80895

UniProt: Q9H0C8