PDIA2 antibody (AA 117-492)

Catalog No. ABIN7599783

The Rabbit Polyclonal anti-PDIA2 antibody has been validated for ELISA, WB and FACS. It is suitable to detect PDIA2 in samples from Human.

Quick Overview for PDIA2 antibody (AA 117-492) (ABIN7599783)

Target: PDIA2 (Protein Disulfide Isomerase Family A, Member 2 (PDIA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDIA2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PDIA2 Antibody

Binding Specificity: AA 117-492

Purpose: Anti-PDIP/PDIA2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDIP/PDIA2 Antibody Picoband® (ABIN7599783). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDIP/PDIA2 recombinant protein (Position: Y117-D492).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Desilva, M. G., Lu, J., Donadel, G., Modi, W. S., Xie, H., Notkins, A. L., Lan, M. S. Characterization and chromosomal localization of a new protein disulfide isomerase, PDIp, highly expressed in human pancreas. DNA Cell Biol. 15: 9-16, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDIA2

Target: PDIA2 (Protein Disulfide Isomerase Family A, Member 2 (PDIA2))

Alternative Name: PDIA2

Background:

Synonyms: Leucine-rich PPR motif-containing protein, mitochondrial, 130 kDa leucine-rich protein, LRP 130, GP130, LRPPRC, LRP130

Tissue Specificity: Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.

Background: Protein disulfide isomerase family A member 2 is a protein that in humans is encoded by the PDIA2 gene. This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels.

Molecular Weight: 65 kDa

Gene ID: 64714

UniProt: Q13087

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Unfolded Protein Response