PDIA2 antibody (AA 477-481)

Catalog No. ABIN768548

The Goat Polyclonal anti-PDIA2 antibody is suitable to detect PDIA2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for PDIA2 antibody (AA 477-481) (ABIN768548)

Target: PDIA2 (Protein Disulfide Isomerase Family A, Member 2 (PDIA2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PDIA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PDIA2 Antibody

Binding Specificity: AA 477-481

Purpose: PDIA2 (aa477-481)

Sequence: EYKSTRDLET FSK

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EYKSTRDLETFSK, from the internal region (near C Terminus) of the protein sequence according to NP_006840.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas shows ER staining in the beta cells of Islets of Lagerhans . Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 65 kDa band observed in Human, Mouse and Rat Pancreas lysates (calculated MW of 58.2 kDa according to NP_006840.2). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDIA2

Target: PDIA2 (Protein Disulfide Isomerase Family A, Member 2 (PDIA2))

Alternative Name: PDIA2

Background: PDIA2, protein disulfide isomerase family A, member 2, LA16c-314G4.2, PDA2, PDI, PDIP, PDIR, OTTHUMP00000067350, OTTHUMP00000197166, Rho GDP dissociation inhibitor gamma, pancreas-specific protein disulfide isomerase, pancreatic protein disulfide isomeras

Molecular Weight: 58.2kDa according to NP_006840.2

Gene ID: 64714, 69191, 287164

NCBI Accession: NP_006840

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Unfolded Protein Response