RIT2 antibody (AA 119-193)

Catalog No. ABIN7599801

The Rabbit Polyclonal anti-RIT2 antibody has been validated for WB, ELISA and FACS. It is suitable to detect RIT2 in samples from Human, Mouse and Rat.

Quick Overview for RIT2 antibody (AA 119-193) (ABIN7599801)

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RIT2 Antibody

Binding Specificity: AA 119-193

Purpose: Anti-RIT2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RIT2 Antibody Picoband® (ABIN7599801). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RIT2 recombinant protein (Position: Q119-K193).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Calissano, M., Latchman, D. S. Functional interaction between the small GTP-binding protein Rin and the N-terminal of Brn-3a transcription factor. Oncogene 22: 5408-5414, 2003. 2. Gross, M. B. Personal Communication. Baltimore, Md. 1/29/2015. 3. Lee, C.-H. J., Della, N. G., Chew, C. E., Zack, D. J. Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of Ras proteins. J. Neurosci. 16: 6784-6794, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RIT2

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Alternative Name: RIT2

Background:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: GTP-binding protein Rit2 is a protein that in humans is encoded by the RIT2 gene. RIN belongs to the RAS (HRAS, MIM 190020) superfamily of small GTPases

Molecular Weight: 17,21 kDa

Gene ID: 6014

UniProt: Q99578

Pathways: Neurotrophin Signaling Pathway