ANKRD1 antibody (AA 120-319)

Catalog No. ABIN7599829

This anti-ANKRD1 antibody is a Rabbit Polyclonal antibody detecting ANKRD1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for ANKRD1 antibody (AA 120-319) (ABIN7599829)

Target: ANKRD1 (Ankyrin Repeat Domain 1 (Cardiac Muscle) (ANKRD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANKRD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ANKRD1 Antibody

Binding Specificity: AA 120-319

Purpose: Anti-ANKRD1 Antibody Picoband®

Characteristics: Anti-ANKRD1 Antibody Picoband® (ABIN7599829). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ANKRD1 recombinant protein (Position: D120-F319). Human ANKRD1 shares 93.5% and 95% amino acid (aa) sequence identity with mouse and rat ANKRD1, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Chu, W., Burns, D. K., Swerlick, R. A., Presky, D. H. Identification and characterization of a novel cytokine-inducible nuclear protein from human endothelial cells. J. Biol. Chem. 270: 10236-10245, 1995. 2. de Waard, V., van Achterberg, T. A. E., Beauchamp, N. J., Pannekoek, H., de Vries, C. J. M. Cardiac ankyrin repeat protein (CARP) expression in human and murine atherosclerotic lesions: activin induces Carp in smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 23: 64-68, 2003. 3. Kanai, H., Tanaka, T., Aihara, Y., Takeda, S., Kawabata, M., Miyazono, K., Nagai, R., Kurabayashi, M. Transforming growth factor-beta/Smads signaling induces transcription of the cell type-restricted ankyrin repeat protein CARP gene through CAGA motif in vascular smooth muscle cells. Circ. Res. 88: 30-36, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ANKRD1

Target: ANKRD1 (Ankyrin Repeat Domain 1 (Cardiac Muscle) (ANKRD1))

Alternative Name: ANKRD1

Background: Ankyrin repeat domain-containing protein 1, or Cardiac ankyrin repeat protein is a protein that in humans is encoded by the ANKRD1 gene also known as CARP. The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system.

Molecular Weight: 36 kDa

Gene ID: 27063

UniProt: Q15327

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Response to DNA Damage Stimulus