NAB1 antibody (AA 120-480)

Catalog No. ABIN7599831

The Rabbit Polyclonal anti-NAB1 antibody has been validated for WB, ICC, IF and ELISA. It is suitable to detect NAB1 in samples from Human and Rat.

Quick Overview for NAB1 antibody (AA 120-480) (ABIN7599831)

Target: NAB1 (NGFI-A Binding Protein 1 (EGR1 Binding Protein 1) (NAB1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA

Product Details anti-NAB1 Antibody

Binding Specificity: AA 120-480

Purpose: Anti-NAB1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NAB1 Antibody Picoband® (ABIN7599831). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NAB1 recombinant protein (Position: A120-K480).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Buitrago, M., Lorenz, K., Maass, A. H., Oberdorf-Maass, S., Keller, U., Shmitteckert, E. M., Ivashchenko, Y., Lohse, M. J., Engelhardt, S. The transcriptional repressor Nab1 is a specific regulator of pathological cardiac hypertrophy. Nature Med. 11: 837-844, 2005. 2. Le, N., Nagarajan, R., Wang, J. Y. T., Svaren, J., LaPash, C., Araki, T., Schmidt, R. E., Milbrandt, J. Nab proteins are essential for peripheral nervous system myelination. Nature Neurosci. 8: 932-940, 2005. 3. Russo, M. W., Sevetson, B. R., Milbrandt, J. Identification of NAB1, a repressor of NGFI-A- and Krox20-mediated transcription. Proc. Nat. Acad. Sci. 92: 6873-6877, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NAB1

Target: NAB1 (NGFI-A Binding Protein 1 (EGR1 Binding Protein 1) (NAB1))

Alternative Name: NAB1

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490

Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Background: NGFI-A-binding protein 1 is a protein that in humans is encoded by the NAB1 gene. Predicted to enable transcription coregulator activity. Predicted to be involved in regulation of transcription, DNA-templated. Predicted to act upstream of or within endochondral ossification, nervous system development, and regulation of epidermis development. Predicted to be located in nucleoplasm. Predicted to be active in nucleus.

Molecular Weight: 56 kDa

Gene ID: 4664

UniProt: Q13506